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DNA Enzymes

Prokaria develops innovative enzymes from it's own extreme sources in Iceland. The key to success is the unrivalled proprietary collection of thermophilic phage genomes.

You can download information about the different products on the page, as well as place orders. Further information about ordering and contact on the bottom of page.



Ice and FirePhosphorylation Kit

The Ice and Fire Phosphorylation kit includes optimized reagents for the 5’;; end labeling of nucleic acids. The novel protocol provides easy "one-step" handling by combining the low temperature activity of thermolabile Shrimp Alkaline Phosphatase (SAP) and the high temperature activity of the thermostable ThermoPhage polynucleotide kinase (PNK).

Available as kit for 50 reactions

Product code IaF170S: 50 reactions  Price EUR 350.00

Order

Download technical sheet (pdf format)


 

ThermoPhageSingle-Stranded DNA Ligase

ThermoPhage single-stranded DNA ligase can effectively ligate single-stranded DNA at elevated temperatures. The enzyme is the only thermostable enzyme of this kind available and works both on single-stranded DNA and RNA. Well suited for various applications including end-to-end ligation of single-stranded DNA and RACE techniques.

Available as 500 units and 1000 units together with 10x reaction buffers

Product code Rlig122S: 500 Units ( 10U/ml )  Price EUR 250.00

Product code Rlig122L: 1000 Units ( 10U/ml )  Price EUR 400.00

Order

Download technical sheet (pdf format)



ThermoPhagePolynucleotide Kinase

ThermoPhage Polynucleotide Kinase is a novel thermoactive kinase catalysing 5’;; phosphorylation of DNA at elevated temperatures and is the only available thermostable enzyme of this kind. The enzyme has very low 3' phosphatase activity. Thermophage™  Polynucleotide Kinase is well suited for various applications including radioactive labelling of nucleic acids.

Available as 500 units and 1000 units together with 10x reaction buffers

Product code Pnk162S: 500 Units ( 10U/ml )  Price EUR 150.00

Product code Pnk162L: 1000 Units ( 25U/ml )  Price EUR 275.00

Order

Download technical sheet (pdf format)



RmaDNA Ligase

Rma DNA ligase from Rhodothermus marinus is a thermostable NAD+ dependent double-stranded DNA ligase with broad temperatures range between 5 and 65°C. The enzyme catalyzes ligation of DNA with high fidelity and is suitable for various applications including gene synthesis.  

Available as 500 units and 2000 units together with 10x reaction buffers

Product code Dlig120S: 500 Units ( 10U/ml ) Price EUR 100.00

Product code Dlig120L: 2000 Units ( 10U/ml ) Price EUR 280.00

Order

Download technical sheet (pdf format)



TscDNA Ligase

Tsc DNA ligase from Thermus scotoductus is a thermostable NAD+ dependent double-stranded DNA ligase with activity at temperatures between 15 and 75°C.

Available as 500 units and 2000 units together with 10x reaction buffers

Product code Dlig119S: 500 Units ( 10U/ml ) Price EUR 100.00

Product code Dlig119L: 2000 Units ( 10U/ml ) Price EUR 280.00

Ordering

Download technical sheet (pdf format)

Order



Purchase of our molecular biology products is easy. We send our products on dry ice by express delivery. Just send us a dated and hand-signed fax indicating code no. and quantity of the desired components to

Snædis Björnsdottir

Fax: +354 422 5002

For questions please write an e-mail (snaedis.bjornsdottir@matis.is)

Please do not forget to add contact phone number, shipment address and billing address.

We charge an extra of EUR 100,00 for shipments to Europe and EUR 130,00 for shipments to USA. For other destinations please inquire. 

Payment:

We accept bank transfer


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