ÖMA: microbiological methods, food
ÖVA: microbiological methods, water
ÖS: microbiological methods, pathogenes
For membrane filtration the detection limit is connected to the amount of sample which is filtrated.
For other parameters on the list the detection limit given reflects the amount of sample which is most often analysed.
Method: Determination of coliform and faecal coliform bacteria, agarmethod
Name: ÖMA 1
Reference: NMKL 44, 6 th ed., 2004, mod, NMKL 125, 4 th ed., 2005, FDA, 2002, chapter 4
Detection limit: 10 in g or 1 in ml
Responsible: Anna Pála Vignisdóttir
Method: Determination of coliform and faecal coliform bacteria, MPN method
Name: ÖMA 2
Reference: NMKL 96, 4th ed., 2009, Compendium 4th ed., 2001, chapter 8 ( 8.71, 8.72, 8.81 )
Detection limit: 3 in g or ml
Responsible: Anna Pála Vignisdóttir
Method: Líftala við 7°, 22° og 30°C, pour plate method
Name: ÖMA 3
Reference: FDA, 2001, chapter 3 ( pour plate ), NMKL 86, 4th ed., 2006, NMKL 74, 3rd ed., 2000
Detection limit: 10 in g or 1 in ml
Responsible: Anna Pála Vignisdóttir
Method: Total count at 7°, 22° and 30°C, spiral plate method with or without overlayer
Name: ÖMA 4.
Reference: FDA, 2001, chapter 3 ( spiral plate ), Oxoid, 1998, MRS agar, Compendium 4th ed., 2001, Chapter 19 ( p.203 ) and 51 ( p. 526 )
Detection limit: 200 in g
Responsible: Anna Pála Vignisdóttir
Method: Enterobacteriaceae
Name: ÖMA 5.
Reference: NMKL 144 3rd ed., 2005
Detection limit: 10 in g or 1 in ml
Responsible: Anna Pála Vignisdóttir
Method: Bacillus cereus, spread plate and spiral plate methods
Name: ÖMA 7
Reference: NMKL 67, 5th ed., 2003 FDA, 2001, chapter 3 ( spread/spiral plate )
Detection limit: 20 in g or 100 in g when a spiralplater is used
Responsible: Margrét Geirsdóttir
Method: Beta-glucuronidase-positive Escherichia coli, MPN-method
Name: ÖMA 8
Reference: ISO/TS 16649-3, 1st ed., 2005, ISO 7218, 1st ed., 2007
Detection limit: < 20 in 100 g
Responsible: Anna Pála Vignisdóttir
Method: Moulds and yeasts, spread plate and spiral plate methods
Name: ÖMA 9
Reference: NMKL 98, 4th ed., 2005 Compendium 4th ed., 2001, chapter 20 FDA, 2001, chapter 3 ( spread/spiral plate )
Detection limit: 20 in g or 200 in g when a spiralplater is used
Responsible: Anna Pála Vignisdóttir.
Method: Coagulase positive staphylococci, spread plate and spiral plate methods
Name: ÖMA 12
Reference: NMKL 66, 5th ed., 2009 FDA, 2001, chapter 3 (spread/spiral plate)
Detection limit: 20 in g or 100 in g when a spiralplater is used
Responsible: Margrét Geirsdóttir
Method: Coliforms, Faecal coliforms, Escherichia coli, MPN-5 tubes
Name: ÖVA 1
Reference: Standard methods, 21st ed., 2005, 9221 B,C,E,F ISO 9308-2: 1990
Detection limit: 3 in ml
Responsible: Hrólfur Sigurðsson
Method: Coliforms, Faecal coliforms and presumptive E.coli, membrane filtration
Name: ÖVA 2
Reference: ISO 9308-1:2000 Standard methods 21st ed,2005, 9222 B and D State of New York DOH. Environmental laboratory approval program certification manual.
Detection limit: 0 in 100 ml
Responsible: Hrólfur Sigurðsson
Method: Total count at 22 °, 35 ° and 37 °C, pour plate method
Name: ÖVA 5
Reference: Standard methods, 21st ed., 2005, 9215 B ISO 6222: 1999
Detection limit: 0 in ml
Responsible: Hrólfur Sigurðsson
Method: Total count at 22 ° 35 ° and 37 °C, membrane filtration
Name: ÖVA 6
Reference: Standard methods, 21st ed, 2005, 9215 D
Detection limit: 0 in 100 ml
Responsible: Hrólfur Sigurðsson
Method: Sulphitreducing clostridia, membrane filtration
Name: ÖVA 7
Reference: ISO/CD 6461-2, 1986 NMKL 56, 4th ed., 2008
Detection limit: 0 in 20 ml
Responsible: Hrólfur Sigurðsson
Method: Pseudomonas aeruginosa. Membrane filtration
Name: ÖVA 8
Reference: IST EN ISO 16266: 2008
Detection limit: 0 in 100 ml
Responsible: Hrólfur Sigurðsson
Method: Total count at 30°C, 5 days incubation,Membrane filtration
Name: ÖVA 9
Reference: Standard methods, 21st ed., 2005, 9215 Ph. Eur. 6.5th ed., 2008, 2.6.12
Detection limit: 0 in 100 ml
Responsible: Hrólfur Sigurðsson
Method: Yeasts and moulds, membrane filtration
Name: ÖVA 10
Reference: Standard methods, 21st ed., 2005, 9215 Ph. Eur. 6.5th ed., 2009, 2.6.12
Detection limit: 0 in 100 ml
Responsible: Hrólfur Sigurðsson
Method: Enterococcus, membrane filtration
Name: ÖVA 12
Reference: ISO 7899-2, 1st ed., 2000
Detection limit: 0 in 100 ml
Responsible: Hrólfur Sigurðsson
Method: Detection of campylobacter
Name: ÖS 1
Reference: NMKL 3rd ed., 2007
Detection limit: Positive / negative in 11 g
Responsible: Margrét Geirsdóttir
Method: Detection of Salmonella
Name: ÖS 2
Reference: NMKL 71, 5th ed., 1999 ISO 6579:2002 Wellcolex- serogroup identification
Detection limit: Positive / negative in 25 g
Responsible: Margrét Geirsdóttir
Method: Detection of Listeria monocytogenes
Name: ÖS 3
Reference: NMKL 136, 4th ed., 2007 and corrections 2008
Detection limit: Positive/ negative in 25 g
Responsible: Margrét Geirsdóttir
Method: Determination of Clostridium perfringens
Name: ÖS 4
Reference: NMKL 95, 5th ed., 2009
Detection limit: 10 in g or 1 in ml
Responsible: Margrét Geirsdóttir
Method: Total count at 30°C 3 days incubation
Name: ÖL 1
Reference: Ph. Eur 6.5th ed. 2009, 2.6.12
Detection limit: 10 in g
Responsible: Anna Pála Vignisdóttir
Method: Moulds, Yeasts at 22°C 5 days incubation
Name: ÖL 2
Reference: Ph. Eur 6.5th ed. 2009, 2.6.12
Detection limit: 20 in g
Responsible: Anna Pála Vignisdóttir
Method: Escherichia coli
Name: ÖL 3
Reference: Ph. Eur 6.5th ed. 2009, 2.6.13
Detection limit: pos/neg in 1 g
Responsible: Anna Pála Vignisdóttir
Method: Salmonella
Name: ÖL 4
Reference: Ph. Eur 6.5th ed. 2009, 2.6.13
Detection limit: pos/neg in 10 g
Responsible: Anna Pála Vignisdóttir
Method: Pseudomonas aeruginosa
Name: ÖL 5
Reference: Ph. Eur 6.5th ed. 2009, 2.6.13
Detection limit: pos/neg in 1 g
Responsible: Anna Pála Vignisdóttir
Method: Staphylococcus aureus
Name: ÖL 6
Reference: Ph. Eur 6.5th ed. 2009, 2.6.13
Detection limit: pos/neg in 1 g
Responsible: Anna Pála Vignisdóttir